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Reagent Details

Adrenoceptor family

Catalogue number: CA200719
Product: β2 antagonist [(±)-alprenolol]
Selectivity: β1: 7.50 | β2: 8.91 | β3: 7.09

 

 
Pharmacophore (±)-Alprenolol-derivative Catalogue Number  Pack Size Price
Molecular Weight 884 CA200719-TK0* 0.05 mg £137.50*
Purity ≥97% CA200719-020 0.2 mg £500
Excitation 633 nm CA200719-050 0.5 mg £1200
Emission 650 nm CA200719-100 1.0 mg £2150
  
* Ordered as part of a TestKit comprising a selection of 4 ligands, listing 4 catalogue numbers ending in -TK0
 

Applications

For imaging at β1 / β2 / β3 adrenoceptors use solutions up to 100 nM.

Pharmacological Validation

The CA200719 ligand was shown to antagonize the activity of the non-selective β agonist, isoprenaline, in three separate recombinant CHO cell lines expressing either the human β1, β2 or β3 receptor and a cyclic AMP-responsive secreted placental alkaline phosphatase (SPAP) reporter gene.

To determine the apparent KD for CA200719 at β1, β2 and β3 receptors, cells were treated with varying concentrations of isoprenaline alone, or in the presence of 1µM CA200719, and the cyclic AMP-induced expression of SPAP measured.

The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of CA200719, compared to the response curve for the agonist alone, for β1, β2 and β3 receptor expressing cell lines.

 



 

 

 

How to Order